The draw for the Leasing.com Trophy Round of 32 will take place at 2pm on Saturday 16th November, live on Sky Sports' Soccer Saturday.
Following the completion of the group stages, Stanley will now face either Mansfield Town, Bolton Wanderers, Wolverhampton Wanderers Under-21s or Doncaster Rovers.
With the Reds securing three wins from three, John Coleman's side are now guaranteed a home tie in the Round of 32 after clinching top spot in Group B.
The draw will be hosted by Sky Sports presenter Julian Waters, with former Crystal Palace forward Clinton Morrison and ex-England midfielder Sue Smith on hand to draw the teams out.
The ball numbers for the draw can be found below:
Northern Section
Draw A - Leicester City Under-21s (1), Accrington Stanley (2), Salford City (3), Port Vale (4), Mansfield Town (13), Bolton Wanderers (14), Wolverhampton Wanderers Under-21s (15), Doncaster Rovers (16)
Draw B - Everton Under-21s (5), Manchester City Under-21s (6), Blackpool (7), Manchester United Under-21s (8), Scunthorpe United (9), Fleetwood Town (10), Tranmere Rovers (11), Shrewsbury Town (12)
Southern Section
Draw A - Colchester United (1), Portsmouth (2), Brighton and Hove Albion Under-21s (3), Walsall (4), Newport County (13), Chelsea Under-21s (14), Stevenage (15), Northampton Town (16)
Draw B - Exeter City (5), Bristol Rovers (6), Milton Keynes Dons (7), Peterborough United (8), Ipswich Town (9), Oxford United (10), Leyton Orient (11), Coventry City (12)
