Aaron O'Connor and Shaun Jeffers scored to condemn Accrington to a 2-0 home defeat against Newport.
The play-off chasing side took the lead when O'Connor met Max Porter's cross to beat Scott Davies at the near post and their lead was doubled in second half stoppage time as Jeffers slotted into an open goal following a slick counter-attack.
John Coleman named an unchanged team from last Saturday's 2-2 draw at home to York City - Sean Maguire had returned from international duty with the Republic of Ireland under-21 side to make the starting XI after debuting for his country the previous evening.
New arrival Tony Warner was named as a substitute, but there was no place in the matchday squad for the other deadline day signing Ryan Jennings, as there was no room for another loan player in the matchday squad.
The visitors were looking to secure themselves in the play-off places and almost took the lead inside a minute as Yan Klukowski's low delivery from the left found Miles Storey, but Dean Winnard was quickly across to clear the danger.
They continued their siege on the home goal with Andy Sandell flicking a header over from a corner and Ryan Jackson's cross needing to be cleared from inside the six-yard box.
After more than half an hour without a shot on target, Piero Mingoia finally brought a save from a goalkeeper, testing Joe Day in the Newport goal. The winger took aim from 25 yards but his swirling effort was palmed clear, although Day didn't appear to know too much about it.
Stanley captain Luke Joyce collected the ball in midfield and drove forward with Josh Windass and Mingoia flanking him but the captain opted to go for goal and fired off-target.
Accrington were now the team in the ascendancy - Kal Naismith played a neat one-two with Terry Gornell but was stretching to get on the end of the return pass and his awkward contact meant the ball tricked wide of the post.
Liverpool loanee Lloyd Jones flicked Windass' floated free-kick into the arms of Day to give the stopper a routine catch as the referee brought an end to the opening half.
Newport were forced into a half-time change as Day had to be replaced by his deputy, Jamie Stephens.
The second half was much like the first with neither side carrying much threat or testing the goalkeepers.
However, after a dull 20 minutes, the deadlock was broken by the visitors in bizarre circumstances.
Storey was threaded through on goal and almost rounded Davies, who sprawled to save and divert the ball out to Porter on the wing. The Newport man whipped a cross in to the near post and Davies was beaten to the ball by O'Connor, who poked the ball through the goalkeeper's legs to squirm into the bottom corner.
Stanley went in search of an equaliser and threatened when Joyce's probing long ball found Gornell, but the striker narrowed the angle for himself and shot straight into the chest of Stephens at the near post.
Substitute Shay McCartan controlled Dean Winnard's cross but could only drag his effort wide from the edge of the penalty area.
Still the Reds pressed for a goal to get them back on level terms. A cross was fizzed into the six-yard box but substitute Jason Gilchrist couldn't connect with the ball and Sandell cleared for Newport.
Coleman's side were trying desperately to find a goal, but were picked off by the Exiles as central defender Ismail Yakubu charged forward from the back to square for Jeffers to roll into the unguarded net.
There was one chance for the Reds to pull a goal back but Stephens spread himself to deny Gilchrist a first Football League goal as Stanley's winless run in League Two stretched to seven games.
Stanley: Davies; Winnard, Jones, Atkinson; Mingoia, Joyce, Crooks (Gilchrist 85), Windass, Naismith; Maguire (McCartan 64); Gornell.
Subs not used: Warner, Hunt, Conneely, Barry, Bruna.
Attendance: 1,111 (95 away)